LOCUS 5014 bp ds-DNA circular 10-OCT-2007 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind complement(4588..4605) /ApEinfo_label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green primer_bind 4556..4576 /ApEinfo_label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green rep_origin 2377..3059 /ApEinfo_label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 CDS 4676..4744 /ApEinfo_label=LacZ alpha /ApEinfo_fwdcolor=cornflower blue /ApEinfo_revcolor=cornflower blue misc_binding 215..237 /ApEinfo_label=LacO /ApEinfo_fwdcolor=cornflower blue /ApEinfo_revcolor=cornflower blue misc_binding 4528..4550 /ApEinfo_label=LacO /ApEinfo_fwdcolor=cornflower blue /ApEinfo_revcolor=cornflower blue CDS 1620..2279 /ApEinfo_label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow misc_feature 258..917 /ApEinfo_label=GST /ApEinfo_fwdcolor=#008080 /ApEinfo_revcolor=#7d0d82 misc_feature 918..941 /ApEinfo_label=Prescission cleavage site /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff80ff misc_feature 946..999 /ApEinfo_label=LXVPmutV /ApEinfo_fwdcolor=#0000a0 /ApEinfo_revcolor=green ORIGIN 1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC 301 AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC 361 GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC 421 TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA 481 TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC 541 TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT 601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG 661 ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT 721 TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA 781 AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT 841 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC 901 ATCCTCCAAA ATCGGATCTG GAAGTTCTGT TCCAGGGGCC CctggGATCC GATCAGTACT 961 TGGCCGCACC ACAGCATCCG TATCAATGGG CTAAGTAACt cgagcggccg catcgtgact 1021 gactgacgat ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 1081 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 1141 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata 1201 gcggagtgta taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta 1261 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 1321 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 1381 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 1441 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 1501 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 1561 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 1621 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag 1681 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 1741 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 1801 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 1861 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 1921 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa 1981 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 2041 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 2101 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 2161 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 2221 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 2281 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 2341 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 2401 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 2461 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 2521 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 2581 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 2641 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 2701 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 2761 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 2821 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 2881 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 2941 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 3001 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 3061 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 3121 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 3181 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt 3241 ttctccttac gcatctgtgc ggtatttcac accgcataaa ttccgacacc atcgaatggt 3301 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga 3361 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg 3421 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag 3481 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac 3541 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg 3601 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag 3661 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca 3721 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct 3781 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta 3841 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc 3901 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg 3961 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact 4021 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca 4081 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt 4141 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct 4201 catgttatat cccgccgtca accaccatca aacaggattt tcgcctgctg gggcaaacca 4261 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc 4321 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc 4381 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 4441 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 4501 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 4561 aacagctatg accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga 4621 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 4681 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 4741 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga 4801 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc 4861 gcccatctac accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga 4921 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg 4981 ccagacgcga attatttttg atggcgttgg aatt //