LOCUS SYNRSV5NEO 5108 bp DNA linear SYN 06-APR-2001
DEFINITION cDNA expression vector RSV.5(neo).
ACCESSION M83237
VERSION M83237.1 GI:209305
KEYWORDS cDNA expression vector.
SOURCE Expression vector RSV.5(neo)
ORGANISM Expression vector RSV.5(neo)
artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5108)
AUTHORS Long,E.O., Rosen-Bronson,S., Karp,D.R., Malnati,M., Sekaly,R.P. and
Jaraquemada,D.
TITLE Efficient cDNA expression vectors for stable and transient
expression of HLA-DR in transfected fibroblast and lymphoid cells
JOURNAL Hum. Immunol. 31 (4), 229-235 (1991)
MEDLINE 92011006
PUBMED 1655683
FEATURES Location/Qualifiers
source 1..5108
/organism="Expression vector RSV.5(neo)"
/db_xref="taxon:118308"
/focus
source 1..29
/organism="unidentified cloning vector"
/db_xref="taxon:45196"
/note="HindIII to BamHI fragment of pUC12."
source 30..872
/organism="Simian virus 40"
/db_xref="taxon:10633"
/note="Bi-directional SV40 termination and
poly-adenylation sequence."
source 873..879
/organism="unidentified cloning vector"
/db_xref="taxon:45196"
/note="Remnant of gpt gene from the cDNA expresion vector
RSV.5(gpt)."
source 880..2197
/organism="Cloning vector pSV2neo"
/db_xref="taxon:31846"
/note="TN5 neomycin-resistance gene from cloning vector
pSV2neo."
source 2198..2542
/organism="Simian virus 40"
/db_xref="taxon:10633"
/note="SV40 early promoter and origin of replication."
source 2543..4580
/organism="Cloning vector pBR322"
/db_xref="taxon:47470"
/note="Ampicillin resistance gene and origin of
replication from pBR322."
source 4581..5108
/organism="Rous sarcoma virus"
/db_xref="taxon:11886"
/note="5' long terminal repeat of Rous Sarcoma virus."
misc_feature 1..29
/function="polylinker"
/evidence=experimental
misc_feature complement(880..2197)
/note="neomycin resistance gene from pSV2neo, putative
mature peptide - contains internal stop codons; putative"
misc_feature 2543..4580
/product="ampicillin resistance"
/function="ampicillin resistance and origin of
replication"
/evidence=experimental
LTR 4581..5108
/standard_name="5'LTR of Rous Sarcoma Virus"
/function="promoter"
/evidence=experimental
BASE COUNT 1291 a 1245 c 1284 g 1288 t
ORIGIN
1 cttgggctgc aggtcgactc tagaggatcc agacatgata agatacattg atgagtttgg
61 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
121 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
181 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
241 caaatgtggt atggctgatt atgatctcta gtcaaggcac tatacatcaa atattcctta
301 ttaacccctt tacaaattaa aaagctaaag gtacacaatt tttgagcata gttattaata
361 gcagacactc tatgcctgtg tggagtaaga aaaaacagta tgttatgatt ataactgtta
421 tgcctactta taaaggttac agaatatttt tccataattt tcttgtatag cagtgcagct
481 ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc tattactaaa cacagcatga
541 ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt cttctacctt tctcttcttt
601 tttggaggag tagaatgttg agagtcagca gtagcctcat catcactaga tggcatttct
661 tctgagcaaa acaggttttc ctcattaaag gcattccacc actgctccca ttcatcagtt
721 ccataggttg gaatctaaaa tacacaaaca attagaatca gtagtttaac acattataca
781 cttaaaaatt ttatatttac cttagagctt taaatctctg taggtagttt gtccaattat
841 gtcacaccac agaagtaagg ttccttcaca aagatccggg gggtgggcga agaactccag
901 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
961 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
1021 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
1081 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
1141 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
1201 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
1261 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc
1321 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
1381 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
1441 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
1501 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
1561 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
1621 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg
1681 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
1741 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
1801 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
1861 catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa
1921 gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
1981 tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg
2041 caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg
2101 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt
2161 tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctttttgcaa aagcctaggc
2221 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
2281 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
2341 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
2401 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact
2461 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
2521 ctaactgaca cacattccac agaggtggca cttttcgggg aaatgtgcgc ggaaccccta
2581 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
2641 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
2701 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
2761 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
2821 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
2881 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
2941 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
3001 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
3061 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
3121 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
3181 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
3241 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
3301 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
3361 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
3421 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
3481 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
3541 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
3601 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
3661 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
3721 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
3781 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
3841 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
3901 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
3961 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
4021 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
4081 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
4141 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
4201 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
4261 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
4321 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
4381 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
4441 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
4501 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
4561 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
4621 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
4681 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtatctgag
4741 gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc
4801 tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt atgcaataca
4861 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
4921 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
4981 acagacaggt ctgacatgga ttggacgaac cactgaattc cgcattgcag agataattgt
5041 atttaagtgc ctagctcgat acaataaacg ccatttgacc attcaccaca ttggtgtgca
5101 cctccaag