We found that the standard restriction buffers described below give more consistent results than those supplied by vendor.

BUFFER LMR MRB HRB XRB
NaCl - 50mM 100mM 150mM
Tris pH 7.5 10mM 10mM 50mM 50mM
MgCl2 10mM 10mM 10mM 10mM
DTT 1mM 1mM 1mM 1mM