We found that the standard restriction buffers described below give more consistent results than those supplied by vendor.
BUFFER
LMR
MRB
HRB
XRB
NaCl
-
50mM
100mM
150mM
Tris pH 7.5
10mM
10mM
50mM
50mM
MgCl
2
10mM
10mM
10mM
10mM
DTT
1mM
1mM
1mM
1mM