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Help for weight - moles (nucleic acids).


Calculation formulas.


Suppose, that 35ng of 4.7kb RNA were diluted in 86µl of H2O.

Insert these data in the form as it is shown below and push the upper "Calculate". You will obtain that it corresponds to 21.87fmol (or 13170342425 molecules). If you want the answer in "pmol", just select this unit and you will obtain: "0.02187pmol".

   

   Size of the nucl. acid [kb]:    
Mw for "olig" [kDa]:   
"weight" --> "mole":
          
  molecules   
"mole" --> "weight":
          
  molecules   

Now, push "Calculate" in "Calculation of the molar concentration". You should obtain 254.3pM. Change volume for 100µl and just click somewhere outside the field (or, again, push "Calculate"): program will give "218.7pM". In the same way the program will recalculate answer after changing of other parameters (weight, volume, size).

The calculations from the known molar quantity are absolutely analogous.

Calculation of the molar concentration:
   in   

 

Calculation of the weight from the molar concentration:
   in   

 

Calculation formulas.

(it is very short descriptions, because we talk about well-known things)

Denote (units of measurement are in brackets):

the relation between these values will be:



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