

> Calculations > Weight - moles (nucleic acids).
Calculation formulas.
(it is very short descriptions, because we talk about well-known things)
Denote (units of measurement are in brackets):
- m [g] - weight of nucleic acid (for example, quantity in microfuge tube) [gram];
- Mwolig [kDa] - molecular weight of oligonucleotide [kilo Dalton].
- <Mw> [Da] - mean molecular weight of one/pair of nucleotides [Dalton].
- base= 324.5Da
- base pair = 649Da
- ribo base = 340.5Da
- Q [mol] - quantity of nucleic acid (in the same tube) [mole];
- N [pieces] - quantity of nucleic acid [pieces];
- c [M] - molar concentration of nucleic acid [Mole]=[mole/litre];
- V [L] - dilution volume [litre];
- L [kb] - length of nucleic acid [in thousands of bases];
- NA - Avogadro constant = 6.022045x1023[1/mole];
the relation between these values will be:
- m[g] = Q[mol] x Mwolig[kDa] x 103
- m[g] = Q[mol] x <Mw>[Da] x L[kb] x 103
- N[pieces] = Q[mol] x NA
- c[M] x V[L] = Q[mol]
"Practical Molecular Biology" http://molbiol.edu.ru;
e-mail: pmb@molbiol.edu.ru